Bio-Nobile ion exchange methods (CM, DEAE)

The weak ion exchange methods in Bio-Nobile QuickPick kits are based on magnetizable cellulose particles coated with carboxymethyl (CM) or diethyl aminoethyl (DEAE) groups. Different types of proteins will bind to the CM or DEAE coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. In typical protein purification using ion exchange chromatography a mixture of many proteins is used as a sample. After unbound molecules are washed away conditions can be stepwise adjusted, such as by altering the pH and salt concentration to release the molecule(s) of interest from the particles.

High quality purifications using ion exchange methods

Not only do QuickPick methods allow high quality purifications, they also allow flexibility in working methods. You can use the QuicPick magnetic tool to concentrate a protein. This is illustrated below for the protein, cytochrome c.
Read more about protein fractionation and purification:

• Fractionation using weak ion exchange
• Proteomics solutions
Technical Note: protein weak ion exchange
Download sample QuickPick™ CM & DEAE purification protocol
Typical performance of the QuickPick™ kits:
CM DEAE
Sample materials
- bacterial cells Cells from 5 ml cultivation lysed in 500 µl wash buffer and diluted 1:5 Cells from 5 ml cultivation lysed in 500 µl wash buffer and diluted 1:5
- yeast cells 500 mg / 1 ml wash buffer 500 mg / 1 ml wash buffer
- cultured cells 106 cells / 0.5 ml wash buffer 106 cells / 0.5 ml wash buffer
- animal tissue 250 mg / 1 ml wash buffer 250 mg / 1 ml wash buffer
- plant tissue 250 mg / 1 ml wash buffer 250 mg / 1 ml wash buffer
Sample volume 300-1000 µl 300-1000 µl
Processing time for
- one sample <10 min <10 min
- four samples 30-40 min 30-40 min
Elution volume per samle 20-100 µl 20-100 µl
Yield 60 ± 6 µg for aprotinin 60 ± 6 µg for BSA
Cytochrome c in 1 mg/ml solution captured on magnetic particles using the QuickPick™ CM kit and resuspended in a smaller volume.