Bio-Nobile glutathione-S-transferase (GST) fusion protein purification

QuickPick GST Magnetic Particles are formed from magnetic agarose particles which provide an extensive porous surface area especially suitable for the purification of recombinant GST-fusion proteins from cell lysates. The transfer of magnetic particles with PickPen technology enables rapid purification of recombinant GST-fusion proteins. The methodology removes the need of using time-consuming column technology.

High quality purifications using QuickPick™ GST kit

The performance of the QuickPick GST kit in purifying a GST-fusion protein is illustrated by the example below in which GST-Btk SH3 (a GST fusion protein of Bruton’s tyrosine kinase SH3 domain) expressed in E. coli was isolated using the QuickPick GST kit. The cells were lysed in 20 mM Tris-HCl buffer, pH 7.0 before purification.
1 = LMW marker
2 = E. coli cell lysate
3-6 = 3µl eluate from 4 parallel samples

Purified GST-Btk SH3 protein on SDS-PAGE gel
Yields obtained using QuickPick™ IMAC and IMAC Plus kits
Sample material
Bacterial cells E. coli recombinant protein expression:
Cells from 3-10 ml cultivation lysed in 800 µl wash buffer
Sample volume 800 µl
Processing time for
- one sample 10 min
- four samples 30-40 min
Elution volume per samle 20-100 µl
Yield 30 ± 3 µg for HT-GST
Purity > 90%